IGEM:PennState/Labbook/NoahJohnson/2007-7-23: Difference between revisions
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*39.5uL Autoclaved dH2O (Enough to make 50uL total) | *39.5uL Autoclaved dH2O (Enough to make 50uL total) | ||
Template DNA Used: Primers: | *Template DNA Used: Primers: | ||
1) 1741015 3398 & 3399 | **1) 1741015 3398 & 3399 | ||
2) 1741017 3400 & 3401 | **2) 1741017 3400 & 3401 | ||
3) 1741018 3406 & 3407 | **3) 1741018 3406 & 3407 | ||
4) 1741019 3408 & 3409 | **4) 1741019 3408 & 3409 | ||
5) 1741020 3402 & 3403 | **5) 1741020 3402 & 3403 | ||
6) 1741021 3404 & 3405 | **6) 1741021 3404 & 3405 | ||
7) 1741011 3412 & 3413 | **7) 1741011 3412 & 3413 | ||
8) 1741023 3410 & 3411 | **8) 1741023 3410 & 3411 | ||
9) 1741005 3416 & 3417 | **9) 1741005 3416 & 3417 | ||
10) (-) 3410 & 3411 | **10) (-) 3410 & 3411 | ||
Revision as of 06:30, 24 July 2007
July 24, 2007
- 5uL 10X Pfx Amplification buffer/ ThermoPol
- 1.5uL 10mM dNTP
- 1uL 50mM MgSO4
- 1.5uL Primer Mix (0.75uL each)
- 1uL Template DNA (10pg-200ng)
- 0.5uL Pfx DNA Polymerase/ TAq Polymerase
- 39.5uL Autoclaved dH2O (Enough to make 50uL total)
- Template DNA Used: Primers:
- 1) 1741015 3398 & 3399
- 2) 1741017 3400 & 3401
- 3) 1741018 3406 & 3407
- 4) 1741019 3408 & 3409
- 5) 1741020 3402 & 3403
- 6) 1741021 3404 & 3405
- 7) 1741011 3412 & 3413
- 8) 1741023 3410 & 3411
- 9) 1741005 3416 & 3417
- 10) (-) 3410 & 3411
