IGEM:PennState/Labbook/NoahJohnson/2007-7-4: Difference between revisions

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Line 14: Line 14:
***13uL H2O
***13uL H2O


*Ran Transformation
*Plated on LB Amp


9:30-3:30
9:30-3:30

Latest revision as of 07:03, 5 July 2007

July 4, 2007

  • Poured gel
  • Ligation (Started at 12:50):
    • (+) 20uL 1741015-PSB1A2
      • 2uL T4 Ligase buffer
      • 4ul Vector (P1010 in PSB1A2+S+E)
      • 12uL Insert (I741015+S+E)
      • 1uL T4 DNA Ligase
      • 1uL H2O
    • (-) 20uL PSB1A2
      • 2uL T4 Ligase buffer
      • 4uL Vector
      • 1uL T4 DNA Ligase
      • 13uL H2O
  • Ran Transformation
  • Plated on LB Amp

9:30-3:30

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