IGEM:PennState/Labbook/NoahJohnson/2007-7-5: Difference between revisions
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**Autoclave 20 mins slow exhaust | **Autoclave 20 mins slow exhaust | ||
**Added | **Added Kanamycin (25uL to 12 plates) | ||
**Added HSL (25uL to 4 of the Kan plates) | |||
**Final [eiken] = 0.28% | **Final [eiken] = 0.28% | ||
*Transformation (Started 12:20) | *Transformation (Started 12:20) | ||
Latest revision as of 12:15, 5 July 2007
July 5, 2007
- Ran Ligation (Started 9:45)
- (+) 20uL 1741015-PSB1A2
- 2uL T4 Ligase buffer
- 4ul Vector (P1010 in PSB1A2+S+E)
- 12uL Insert (I741015+S+E)
- 1uL T4 DNA Ligase
- 1uL H2O
- (-) 20uL PSB1A2
- 2uL T4 Ligase buffer
- 4uL Vector
- 1uL T4 DNA Ligase
- 13uL H2O
- (+) 20uL 1741015-PSB1A2
- Made Motility Plates
- 0.25g tryptone
- 0.2g NaCl
- 0.07g eiken agar
- 0.15g sodium citrate
- 25mL water
- Autoclave 20 mins slow exhaust
- Added Kanamycin (25uL to 12 plates)
- Added HSL (25uL to 4 of the Kan plates)
- Final [eiken] = 0.28%
- Transformation (Started 12:20)
