IGEM:PennState/Labbook/NoahJohnson/2007-7-5

July 5, 2007

• Ran Ligation (Started 9:45)
  • (+) 20uL 1741015-PSB1A2
    • 2uL T4 Ligase buffer
    • 4ul Vector (P1010 in PSB1A2+S+E)
    • 12uL Insert (I741015+S+E)
    • 1uL T4 DNA Ligase
    • 1uL H2O
  • (-) 20uL PSB1A2
    • 2uL T4 Ligase buffer
    • 4uL Vector
    • 1uL T4 DNA Ligase
    • 13uL H2O

• Made Motility Plates
  • 0.25g tryptone
  • 0.2g NaCl
  • 0.07g eiken agar
  • 0.15g sodium citrate
  • 25mL water

• • Autoclave 20 mins slow exhaust
  • Added kanamycin
  • Final [eiken] = 0.28%