IGEM:PennState/Labbook/NoahJohnson/2007-7-5

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July 5, 2007

  • Ran Ligation (Started 9:45)
    • (+) 20uL 1741015-PSB1A2
      • 2uL T4 Ligase buffer
      • 4ul Vector (P1010 in PSB1A2+S+E)
      • 12uL Insert (I741015+S+E)
      • 1uL T4 DNA Ligase
      • 1uL H2O
    • (-) 20uL PSB1A2
      • 2uL T4 Ligase buffer
      • 4uL Vector
      • 1uL T4 DNA Ligase
      • 13uL H2O
  • Made Motility Plates
    • 0.25g tryptone
    • 0.2g NaCl
    • 0.07g eiken agar
    • 0.15g sodium citrate
    • 25mL water
    • Autoclave 20 mins slow exhaust
    • Added Kanamycin (25uL to 12 plates)
    • Added HSL (25uL to 4 of the Kan plates)
    • Final [eiken] = 0.28%


  • Transformation (Started 12:20)
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