IGEM:PennState/Labbook/NoahJohnson/2007-7-6: Difference between revisions
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**Well 2- (-) Pfx | **Well 2- (-) Pfx | ||
**Well 3- 56°C Pfx | **Well 3- 56°C Pfx | ||
**Well 4- | **Well 4- 57.1°C Pfx | ||
**Well 5- 58. | **Well 5- 58.6°C Pfx | ||
**Well 6- | **Well 6- 61.3°C Pfx | ||
**Well 7- | **Well 7- 64.9°C Pfx | ||
**Well 8- | **Well 8- 67.5°C Pfx | ||
**Well 9- | **Well 9- 69.1°C Pfx | ||
**Well 10- | **Well 10- 70°C Pfx | ||
Latest revision as of 12:27, 6 July 2007
July 6, 2007
- Yesterdays motility plates found that motility was greatest at 26°C so today we are running 5 motility trials at 26°C with varying concentrations of eiken agar
- Made motility plates
- 0.25g tryptone
- 0.2g NaCl
- 0.15g sodium citrate
- 25mL water
- Eiken Agar:
- 4 plates of 0.24%
- 4 plates of 0.26%
- 4 plates of 0.28%
- 4 plates of 0.30%
- 4 plates of 0.32%
- Ran Gel on Pfx PCR products (Start 2:00)
- Used Sybr-green
- Well 1- DNA Ladder
- Well 2- (-) Pfx
- Well 3- 56°C Pfx
- Well 4- 57.1°C Pfx
- Well 5- 58.6°C Pfx
- Well 6- 61.3°C Pfx
- Well 7- 64.9°C Pfx
- Well 8- 67.5°C Pfx
- Well 9- 69.1°C Pfx
- Well 10- 70°C Pfx
