IGEM:PennState/Labbook/NoahJohnson/2007-8-2: Difference between revisions
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Protocol: | Protocol: | ||
# Take 200mL sterile water and supplement with 25mL | # Take 200mL sterile water and supplement with 25mL of 12.5% sugar solution | ||
*To make 12.5% sugar solution add 0.5g glucose/xylose to 25mL of sterile water and filter-sterilize | |||
# Add | |||
10:30- | 10:30- | ||
Revision as of 15:03, 2 August 2007
August 2, 2007
- Ordered UV cuvettes
- Began growing up 6 3ml cultures of crp* in LB
- Will TELT dna prep them tonight or tomorrow
- Prepared M9 Minimal Media (250mL each):
- 0.2% glucose
- 0.2% glucose + 0.2% xylose
- 0.2% xylose
- 0.2* glycerol (- control)
Protocol:
- Take 200mL sterile water and supplement with 25mL of 12.5% sugar solution
- To make 12.5% sugar solution add 0.5g glucose/xylose to 25mL of sterile water and filter-sterilize
- Add
10:30-
