IGEM:PennState/Labbook/NoahJohnson/2007-8-2: Difference between revisions
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[http://www.thelabrat.com/protocols/m9minimal.shtml Another protocol] | [http://www.thelabrat.com/protocols/m9minimal.shtml Another protocol] | ||
[http://www.madsci.org/~lynn/micro/techniques/pour_plates/MINagar.html Protocol for plates] | |||
10:30- | 10:30- |
Revision as of 15:31, 2 August 2007
August 2, 2007
- Ordered UV cuvettes
- Began growing up 6 3ml cultures of crp* in LB
- Will TELT dna prep them tonight or tomorrow
M9 Minimal Media (250mL each):
- 0.2% glucose
- 0.2% glucose + 0.2% xylose
- 0.2% xylose
- 0.2* glycerol (- control)
For 250mL of media:
- 200mL sterile water
- 25mL sugar solution
- Add 0.5g glucose/xylose to 25mL sterile water
- Filter-sterilize before adding
- Makes a 12.5% sugar solution and a 0.2% overall glucose/xylose media
- 50mL of 5X M9 salts
- 12.8g Na2HP04*7H2O
- 3g KH2PO4
- 0.5g NaCl
- 1g NH4Cl
- Dissolve in 200mL deionized water and autoclave to sterilize
- 500 uL 1M MgSO4
- Dissolve 24.65g MgSO4*7H20 in 100mL H20
- Autoclave to sterilize
- 25uL 1M CaCl2
- Dissolve 14.7 g CaCl2*2H2O in 100mL H2O
- Autoclave to sterilize
Another protocol
Protocol for plates
10:30-