IGEM:PennState/Labbook/NoahJohnson/2007-8-21: Difference between revisions
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<h1>August 21, 2007</h1> | <h1>August 21, 2007</h1> | ||
*Set up 20uL double digests with psB1A2 as well as sequential digests with EcoR1 and SpeI | |||
*Used MP from galen's box marked: | |||
**6B for E+S | |||
**4B for E | |||
**3B for S | |||
*Started incubating at 11:03am | |||
*Ran gel on restriction products | |||
*Started @ 1:50pm | |||
*Started an overnight ligation at 16C with old crp* 4uL restriction E+S from yesterday and the gel extracted psB1A2 restriction E+S from 8/10 | |||
*Used a 6:2 ins:vect ratio | |||
9:30-3:30 | 9:30-3:30 | ||
Revision as of 09:30, 22 August 2007
August 21, 2007
- Set up 20uL double digests with psB1A2 as well as sequential digests with EcoR1 and SpeI
- Used MP from galen's box marked:
- 6B for E+S
- 4B for E
- 3B for S
- Started incubating at 11:03am
- Ran gel on restriction products
- Started @ 1:50pm
- Started an overnight ligation at 16C with old crp* 4uL restriction E+S from yesterday and the gel extracted psB1A2 restriction E+S from 8/10
- Used a 6:2 ins:vect ratio
9:30-3:30
