IGEM:PennState/Labbook/NoahJohnson/2007-9-20: Difference between revisions
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Latest revision as of 20:40, 20 September 2007
September 20, 2007
- DH5a competent cell prep
- Ligation labelling system:
- 1: Tube 1 crp* & Tube 1 psB1A2 (Fridge ligation)
- 2: Tube 2 crp* & Tube 4 psB1A2 (Fridge ligation)
- 3: (-) Tube 1 psB1A2 (Fridge ligation)
- 4: Tube 3 crp* & Tube 1 psB1A2 (Benchtop ligation)
- 5: Tube 4 crp* & Tube 4 psB1A2 (Benchtop ligation)
- 6: (-) Tube 4 psB1A2 (Benchtop ligation)
- Transformation:
- 3uL ligation mixture in 50uL DH5a competent cells
- Will do 2 transformations for each ligation, one with todays competent cells (New) and one with yesterday's (Old).
- Plated on LB Amp
- Made 500mM filter-sterilized glucose soln for SOC:
- 0.9g Glucose (MW=180.16 g/mol) added to 10mL dH20
- Filter sterilized and put in 15mL Falcon tube
- Can be found in rack in fume hood
6-11:30
