IGEM:PennState/Labbook/NoahJohnson/2007-9-20

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Revision as of 23:40, 20 September 2007 by Nrj5011 (Talk | contribs)
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September 20, 2007

  • DH5a competent cell prep
  • Ligation labelling system:
    • 1: Tube 1 crp* & Tube 1 psB1A2 (Fridge ligation)
    • 2: Tube 2 crp* & Tube 4 psB1A2 (Fridge ligation)
    • 3: (-) Tube 1 psB1A2 (Fridge ligation)
    • 4: Tube 3 crp* & Tube 1 psB1A2 (Benchtop ligation)
    • 5: Tube 4 crp* & Tube 4 psB1A2 (Benchtop ligation)
    • 6: (-) Tube 4 psB1A2 (Benchtop ligation)
  • Transformation:
    • 3uL ligation mixture in 50uL DH5a competent cells
    • Will do 2 transformations for each ligation, one with todays competent cells (New) and one with yesterday's (Old).
    • Plated on LB Amp
  • Made 500mM filter-sterilized glucose soln for SOC:
    • 0.9g Glucose (MW=180.16 g/mol) added to 10mL dH20
    • Filter sterilized and put in 15mL Falcon tube
    • Can be found in rack in fume hood

6-11:30

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