IGEM:PennState/Labbook/NoahJohnson/2007-9-3: Difference between revisions
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*Ran gel on restrictions to extract psB1A2 and confirm that I still have crp* | *Ran gel on restrictions to extract psB1A2 and confirm that I still have crp* | ||
12- | *Ran out of TAE buffer and Tien's lab is closed and locked. Will get more TAE and re-restrict tomorrow or wednesday. | ||
12-7 | |||
Latest revision as of 16:30, 5 September 2007
September 3, 2007
- Ran gel on PCR products
- PCR purified the 2 PCR rxns which worked:
- 0.01uL crp* template Pfu Polymerase
- 1.0uL crp* template Phusion Polymerase
- Restricted the 2 PCR purified samples along with P1010 in psB1A2
- Cut with E+S, 20uL digests
- Ran gel on restrictions to extract psB1A2 and confirm that I still have crp*
- Ran out of TAE buffer and Tien's lab is closed and locked. Will get more TAE and re-restrict tomorrow or wednesday.
12-7
