IGEM:PennState/Labbook/NoahJohnson/2007-9-5: Difference between revisions
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**cut with EcoR1 and SpeI | **cut with EcoR1 and SpeI | ||
**Started incubating at 6:45pm | **Started incubating at 6:45pm | ||
*Ran gel on restriction products and gel extracted psB1A2 | |||
6-11 | 6-11 | ||
Latest revision as of 15:32, 6 September 2007
September 5, 2007
- Made more 1X TAE buffer
- Added 40mL of 50X TAE buffer to 4 L flask
- Added 1960mL dH20 and mixed
- Restriction digest on crp* and P1010 in psB1A2
- cut with EcoR1 and SpeI
- Started incubating at 6:45pm
- Ran gel on restriction products and gel extracted psB1A2
6-11
