IGEM:PennState/Labbook/NoahJohnson/2007-9-7: Difference between revisions
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(New page: <h1>September 7, 2007</h1> *Weekend plan: **Friday evening: Transform into DH5a, plate on LB Amp, Also innoculate culture of DH5a **Saturday: Innoculate cultures from plates if transformat...) |
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**Saturday: DH5a competent cell prep if unsuccessful, followed by transformation. | **Saturday: DH5a competent cell prep if unsuccessful, followed by transformation. | ||
* | *Transformed crp* into DH5a. Only had enough competent cells for 6 transformations | ||
* | **Transformed Ligation #: 1,4,6,7,9,10 | ||
**Plated on LB Amp and put in 47C incubator overnight | |||
10:30-12:30am | 10:30-12:30am | ||
3:30-6pm | |||
Revision as of 13:44, 7 September 2007
September 7, 2007
- Weekend plan:
- Friday evening: Transform into DH5a, plate on LB Amp, Also innoculate culture of DH5a
- Saturday: Innoculate cultures from plates if transformation is successful.
- Saturday: DH5a competent cell prep if unsuccessful, followed by transformation.
- Transformed crp* into DH5a. Only had enough competent cells for 6 transformations
- Transformed Ligation #: 1,4,6,7,9,10
- Plated on LB Amp and put in 47C incubator overnight
10:30-12:30am 3:30-6pm
