IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/04/22: Difference between revisions
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Anze Vozelj (talk | contribs) (Autocreate 2015/04/22 Entry for IGEM:Slovenia_HS_2015/2009/Notebook/Test) |
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= | =Gene isolation= | ||
* | *We have ordered specific oligonucleotides (BdhA, CtfAB).Our goal was the isolation of genes reffering to ''Clostridium acetobutylicum''. There is a table beneath. As oligonucleotides we have used BdhA (R), Bdha (F), ctfAB (R), ctfAB (F), 16S (F), 16S (R). Basically we were changing only the source of isolation. | ||
Isolation sources: | |||
*broth | |||
*10x diluted broth | |||
* DNA | |||
* 100x diluted DNA | |||
==== Mixture for PCR reaction (master mix) ==== | |||
::{| border="1" cellpadding="2" cellspacing="0" | |||
|- | |||
!component||||quantity (µL)|| | |||
|- | |||
|oligo 1 (10µM)||1||10|| | |||
|- | |||
|oligo 2 (10µM)||1||10|| | |||
|- | |||
|dNTP (2mM)||1 ||10|| | |||
|- | |||
|MgCl2||2||20|| | |||
|- | |||
|buffer||2||20|| | |||
|- | |||
|dH2O||11||110|| | |||
|- | |||
|Vtot.||/||190|| | |||
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Revision as of 07:29, 1 May 2015
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Gene isolation
Isolation sources:
Mixture for PCR reaction (master mix)
|