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- [FILLER; PLEASE REPLACE:] The objective of this year’s project is to develop a system of devices that maintains the equilibrium of Th17 and Treg cell levels in vitro. Treg cells, short for regulatory T cells, are key regulators of immune responses to self-tissues and infectious agents. Th17 cells are a subset of the helper T cells, recognized as one of the master mediators of inflammation and immune activation in a variety of pathological conditions. Th17 cells have particularly been associated with a number of autoimmune diseases and complications, such as rheumatoid arthritis, respiratory diseases, allograft rejection, systemic lupus erythematoisis, and multiple sclerosis. Treg normally inactivate self-reactive immune cells and regulate normal immune responses but during diseases such as cancer and HIV can be over-stimulated and open the patient up for opportunistic infections. We intend to develop a device that can sense imbalances in Th17 and Treg cell numbers and secrete factors that restore the cell populations to homeostatic levels. Our device will be engineered to specifically target Th17-induced inflammatory bowel disease and hyperactivity of Tregs in colorectal cancer. What is exciting/unique about this project is that it will serve as a proof that probiotic devices can be designed that not only treat disease states but can dynamically sense disease environments spatially and temporally. We have high chances of succeeding because our project will integrate past research into a cohesive device - building off of rigorous scientific investigation. Moreover, Stanford faculty members, leaders in the bioengineering business, and our graduate advisors constantly vet the progress of our project.
Team Weekly Descriptions
- Week July 20th-24th
- Lab Safety Training w/ Isis and Jerome for all team members and familiarity w/ proocols
- All transformations of RBS and Terminators done .... everything possible w/out the genes
- Get wiki templates created
- Parse modeling project and assign team members.
- Week July 27th-31st
- Device 1: cloning steps 1a,b,c, 2a
- device 2: cloning steps 1d?
- Make competent cells: Tuesday 28th July, Endy lab, 11am. Need 2 people.
- Design a simple and robust assay for SoxR activation of Soxs promoter (using the GFP generator).
- Look into a detection assay for Retinol from Beta-carotene and BLH (HPLC, Smolke lab).
Notes and Comments
- [Please leave comments here!!!]
Individual Member Notebooks