IGEM:Stanford/2009/Notebook/Homeostasis/2009/07/22

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Entries

Daily Work

Goals

  • Familiarity w/ protocols for mini-preps and transformations
  • Successful mini-prep concentrations


Project/Accomplishments

  • Two rounds of mini-preps - both had low results
Mini-Prep 2 w/ Epoch Kit







  • One Round of transformations
Transformation Data






Problems

  • Low concentrations/ poor results


Alternative Solutions

  • Get more familiar w/ lab techniques
  • Switch away from kit


Protocols

Events

  • Jerome mini-prep review 11:30am
  • Speaker Bernard Palsson Clark Center S360 2pm
  • Modeling Presentation 3:15pm
  • Isis transformations and redo of mini-preps 3:30pm

Comments

  • The mini-prep concentrations were very low....

Weekly Goals

  • We need to get as many transformations and as much lab work done as possible this week without the genes
  • [enter the team's weekly goals]



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