IGEM:Stanford/2009/Notebook/Homeostasis/2009/08/04: Difference between revisions

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**Looks like success for some first rounds of cloning!!!
**Looks like success for some first rounds of cloning!!!
***Now we have to do colony PCR to check....
***Now we have to do colony PCR to check....
***Now we have to do colony PCR to check....
***Ligation for 1b
 
**Tonight
***restreaking
***Liquid culture for stuff we are running low on (SoxR, BLH, etc)
***Ran gel from colony PCR
****Re-run in the morning (still getting used to new equipment and it looked funny)
{|
{|
| [[Image:1a.JPG |thumb|left |Results from 1a cloning ]]
| [[Image:1a.JPG |thumb|left |Results from 1a cloning ]]
Line 44: Line 50:


==Events==
==Events==
*Graduate Student Board Meeting at 9am
*'''Graduate Student Board Meeting at 9am'''
**Feedback
**Feedback
***Better Intro
***Change the first slide, it is scary. We could put into in other parts of the powerpoint
***Change the first slide, it is scary. We could put into in other parts of the powerpoint
***Too much text on the second slide
***Too much text on the second slide
***Visual of differentiation of T-cells
***The Movie
***The Movie
****Is it necessary?
**** exaggerate T-cell populations
**** possibly show a graph
****Take out what you are not using... Is it necessary?
****More contrast between before and after the implantation of the device
****More contrast between before and after the implantation of the device
****Take out the green dots
****Take out the green dots
***Think more about the purpose of our projest
***Think more about the purpose of our projest
****How is it different from pre-existing medicine
****How is it different from pre-existing medicine
****What makes it great?
***Explain the location and environment/ symptoms of IBD
***Naming= "NO sensor" "Trp sensor" explain RA- be VERY clear and simple
***How is the device used to treat the disease? Why E. coli? What are the results?
****'''Why is this a good solution?''' - greater context of what you are doing and '''AWESOMENESS!'''
****Sell it!
****Sell it!
***The device level diagram slide
***The device level diagram slide
Line 60: Line 73:
****Reduce the amount of details
****Reduce the amount of details
**Things to think about
**Things to think about
***How many background do we need to give?  
***How many background do we need to give? Background vs. Specifics in Final Presentation
***How many people does this disease effect?
***How many people does this disease effect?
***Talk more about the consequences of implanting the device
***Talk more about the consequences of implanting the device
***Keep all information simple, imagine your audience as really really lazy people
***Keep all information simple, imagine your audience as really really lazy people
***Impress your audience vs. communication
***vBOL--talk more about what it is and what it could be used for
**Slideshow general
***3 concepts per slide
***Show the results, not the process
***'''Inroduce vBOL symbols from the start and then use them throughout the presentation'''
**Problems that we have been having
***A biobrick standard that doesn't use Pst1 because the presence of restriction sites in gene sequence (Trp operon) that we can't mutate
***Assign grad students for assay design
***Trp operon--Meeting with Yanofsky


==Comments==
==Comments==
*[leave a comment!]
*[leave a comment!]
 
http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning


==Weekly Goals==
==Weekly Goals==

Revision as of 10:01, 5 August 2009

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Entries

Daily Work

Goals

  • Ligate, transform, colony PCR...


Project/Accomplishments

  • Wet-Lab
    • Looks like success for some first rounds of cloning!!!
      • Now we have to do colony PCR to check....
      • Ligation for 1b
    • Tonight
      • restreaking
      • Liquid culture for stuff we are running low on (SoxR, BLH, etc)
      • Ran gel from colony PCR
        • Re-run in the morning (still getting used to new equipment and it looked funny)
Results from 1a cloning
Results from 1c cloning
Results from 1d cloning




  • Dry-Lab
    • [what projects occurred today?]
    • [what were the results?]


Problems

  • [what problems occurred? frustrations?]


Alternative Solutions

  • [solutions to the problems/future ideas]

Events

  • Graduate Student Board Meeting at 9am
    • Feedback
      • Better Intro
      • Change the first slide, it is scary. We could put into in other parts of the powerpoint
      • Too much text on the second slide
      • Visual of differentiation of T-cells
      • The Movie
        • exaggerate T-cell populations
        • possibly show a graph
        • Take out what you are not using... Is it necessary?
        • More contrast between before and after the implantation of the device
        • Take out the green dots
      • Think more about the purpose of our projest
        • How is it different from pre-existing medicine
      • Explain the location and environment/ symptoms of IBD
      • Naming= "NO sensor" "Trp sensor" explain RA- be VERY clear and simple
      • How is the device used to treat the disease? Why E. coli? What are the results?
        • Why is this a good solution? - greater context of what you are doing and AWESOMENESS!
        • Sell it!
      • The device level diagram slide
        • Divide into two different slides
        • Reduce the amount of details
    • Things to think about
      • How many background do we need to give? Background vs. Specifics in Final Presentation
      • How many people does this disease effect?
      • Talk more about the consequences of implanting the device
      • Keep all information simple, imagine your audience as really really lazy people
      • Impress your audience vs. communication
      • vBOL--talk more about what it is and what it could be used for
    • Slideshow general
      • 3 concepts per slide
      • Show the results, not the process
      • Inroduce vBOL symbols from the start and then use them throughout the presentation
    • Problems that we have been having
      • A biobrick standard that doesn't use Pst1 because the presence of restriction sites in gene sequence (Trp operon) that we can't mutate
      • Assign grad students for assay design
      • Trp operon--Meeting with Yanofsky

Comments

  • [leave a comment!]

http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning

Weekly Goals

  • [enter the team's weekly goals]



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