IGEM:Stanford/2010/Notebook/11 May 2010: Difference between revisions
Gregory Owen (talk | contribs) No edit summary |
mNo edit summary |
||
| (9 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
{{Template:Stanford_Main_Menu_0910}} | |||
<div style="width:720px;text-align:justify"> | |||
==May 11 2010== | |||
==Project Ideas Poll== | ==Project Ideas Poll== | ||
Hi everyone: | Hi everyone: | ||
| Line 7: | Line 12: | ||
=Brainstorm/Notes= | =Brainstorm/Notes= | ||
Preterm Labor: | |||
- one sign of preterm labor is an increase in pH above 4.5, indicating the presence of bacteria (bacterial vaginosis) | - one sign of preterm labor is an increase in pH above 4.5, indicating the presence of bacteria (bacterial vaginosis) | ||
* ChvG/ChvI moderates acid-inducible genes in agrobacterium (A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid) | * ChvG/ChvI moderates acid-inducible genes in agrobacterium (A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid) | ||
- labor induced by changes in expression of progesterone ''receptors'', not the steroid | - labor induced by changes in expression of progesterone ''receptors'', not the steroid itself (Progesterone Withdrawal and Estrogen Activation in Human Parturition Are Coordinated by Progesterone Receptor A Expression in the Myometrium) | ||
- cervical length seems to be an important risk factor | |||
- http://jcem.endojournals.org/cgi/content/abstract/94/6/2066 | |||
Concrete: | |||
-Caulobacter crescentus produces a strong glue-like secretion, has been fully sequenced | |||
==References brought up during meeting== | |||
'''Temporal Signal Integration''' | |||
<biblio> | |||
#friedland pmid=19478183 | |||
</biblio> | |||
Second generation genetic circuit review: | |||
<biblio> | |||
#lu pmid=20010597 | |||
</biblio> | |||
Biological Networks Review | |||
http://arjournals.annualreviews.org/doi/pdf/10.1146/annurev.bioeng.3.1.391 | |||
=For Next Time= | |||
Every team member should research the following: | |||
* possible gene circuit designs | |||
* existing networks in nature | |||
* compelling applications for ratiometric sensors other than preterm labor | |||
* potential reporters (these will probably be application-specific ie. GFP wouldn't work for preterm labor) | |||
Additionally, you should come up with a list of objectives that will need to be completed and rank them in order of importance. | |||
To aid in all of the above, each team member should meet with a grad student by next time. Email one of them to set up a meeting. | |||
Latest revision as of 16:26, 16 May 2010
<html> <head> <style type="text/css"> body{background:#FFFFCC} table.menu{ background:#990000; padding:5px; color:black; } font.cell{ font-family:Verdana; font-size:14px; font-style:normal; color:#ffffff; width:99px; text-align:center; padding:0px } font.cell:hover{font-weight:bold; cursor:pointer; text-decoration:none } img{padding-bottom:5px} a.menu{text-decoration:none; color:white} a.menu:visited{text-decoration:none; color:white} </style> </head> </html>
|
May 11 2010
Project Ideas Poll
Hi everyone:
Please choose three ideas from the following list by Wednesday 11:59. When deciding, consider the feasibility, complexity, and your interest in the topic. The top three ideas will then be heavily researched, and will most likely form the basis of the actual project. Also feel free to add any other ideas that are not on the poll.
http://spreadsheets.google.com/ccc?key=0AoaiDJQdUZSYdFkyQWN4WXlUbk95N3BXVGFhejlJTkE&hl=en
Brainstorm/Notes
Preterm Labor:
- one sign of preterm labor is an increase in pH above 4.5, indicating the presence of bacteria (bacterial vaginosis)
- ChvG/ChvI moderates acid-inducible genes in agrobacterium (A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid)
- labor induced by changes in expression of progesterone receptors, not the steroid itself (Progesterone Withdrawal and Estrogen Activation in Human Parturition Are Coordinated by Progesterone Receptor A Expression in the Myometrium)
- cervical length seems to be an important risk factor
- http://jcem.endojournals.org/cgi/content/abstract/94/6/2066
Concrete:
-Caulobacter crescentus produces a strong glue-like secretion, has been fully sequenced
References brought up during meeting
Temporal Signal Integration
- Friedland AE, Lu TK, Wang X, Shi D, Church G, and Collins JJ. Synthetic gene networks that count. Science. 2009 May 29;324(5931):1199-202. DOI:10.1126/science.1172005 |
Second generation genetic circuit review:
- Lu TK, Khalil AS, and Collins JJ. Next-generation synthetic gene networks. Nat Biotechnol. 2009 Dec;27(12):1139-50. DOI:10.1038/nbt.1591 |
Biological Networks Review http://arjournals.annualreviews.org/doi/pdf/10.1146/annurev.bioeng.3.1.391
For Next Time
Every team member should research the following:
- possible gene circuit designs
- existing networks in nature
- compelling applications for ratiometric sensors other than preterm labor
- potential reporters (these will probably be application-specific ie. GFP wouldn't work for preterm labor)
Additionally, you should come up with a list of objectives that will need to be completed and rank them in order of importance.
To aid in all of the above, each team member should meet with a grad student by next time. Email one of them to set up a meeting.
