IGEM:Stanford/2010/Notebook/15 April 2010: Difference between revisions
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==Journal Club 4/15/10== | ==Journal Club 4/15/10== | ||
[http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ... | [http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ... | ||
===DNA-ligand binding=== | |||
d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L] | |||
If we define: | |||
[TotalDNA] = [DNA] + [DNA-L] | |||
Then: | |||
d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L] | |||
Solving, we get: | |||
[DNA-L] = [TotalDNA][L]/(k_on/k_off + L) | |||
Revision as of 21:25, 15 April 2010
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Journal Club 4/15/10
Presentation by ...
DNA-ligand binding
d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]
If we define: [TotalDNA] = [DNA] + [DNA-L]
Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]
Solving, we get: [DNA-L] = [TotalDNA][L]/(k_on/k_off + L)
