# IGEM:Stanford/2010/Notebook/15 April 2010

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 Revision as of 19:35, 13 April 2010 (view source)m ← Previous diff Revision as of 00:25, 16 April 2010 (view source) (DNA-ligand binding)Next diff → Line 3: Line 3: ==Journal Club 4/15/10== ==Journal Club 4/15/10== [http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ... [http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ... + + ===DNA-ligand binding=== + + d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L] + + If we define: + [TotalDNA] = [DNA] + [DNA-L] + + Then: + d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L] + + Solving, we get: + [DNA-L] = [TotalDNA][L]/(k_on/k_off + L)

## Journal Club 4/15/10

Presentation by ...

### DNA-ligand binding

d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]

If we define: [TotalDNA] = [DNA] + [DNA-L]

Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]

Solving, we get:

[DNA-L] = [TotalDNA][L]/(k_on/k_off + L)