IGEM:Stanford/2010/Notebook/15 April 2010

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
(DNA-ligand binding)
Line 3: Line 3:
==Journal Club 4/15/10==
==Journal Club 4/15/10==
[http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ...
[http://docs.google.com/present/edit?id=0AeGtz3V56Y9xZGRtOGc0dm1fMTIwNWNqeG03enBn&hl=en Presentation] by ...
 +
 +
===DNA-ligand binding===
 +
 +
d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]
 +
 +
If we define:
 +
[TotalDNA] = [DNA] + [DNA-L]
 +
 +
Then:
 +
d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]
 +
 +
Solving, we get:
 +
[DNA-L] = [TotalDNA][L]/(k_on/k_off + L)

Revision as of 23:25, 15 April 2010

Journal Club 4/15/10

Presentation by ...

DNA-ligand binding

d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]

If we define: [TotalDNA] = [DNA] + [DNA-L]

Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]

Solving, we get:

[DNA-L] = [TotalDNA][L]/(k_on/k_off + L)
Personal tools