IGEM:Stanford/2010/Notebook/15 April 2010
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So we end up with: | So we end up with: | ||
[DNA-L] = [TotalDNA][L] / (kD + L) | [DNA-L] = [TotalDNA][L] / (kD + L) | ||
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| + | ===See also=== | ||
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| + | * [http://www.nature.com/nature/journal/v463/n7279/full/463301a.html Commentary on Danino et al.] | ||
Revision as of 00:35, 16 April 2010
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Journal Club 4/15/10
Presentation by ...
DNA-ligand binding
d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]
If we define: [TotalDNA] = [DNA] + [DNA-L]
Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]
Solving, we get: [DNA-L] = [TotalDNA][L] / (k_off/k_on + L)
Usually, we define the dissociation constant to be: kD = k_off/k_on
So we end up with: [DNA-L] = [TotalDNA][L] / (kD + L)
