IGEM:Stanford/2010/Notebook/15 April 2010

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Journal Club 4/15/10)
(Repressilator)
Line 33: Line 33:
The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper].
The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper].
-
[[wikipedia:Repressilator]] on Wikipedia.
+
 
 +
[[wikipedia:Repressilator|Repressilator]] on Wikipedia.
===See also===
===See also===

Revision as of 00:40, 16 April 2010

Journal Club 4/15/10

Presentation by ...

DNA-ligand binding

d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]

If we define: [TotalDNA] = [DNA] + [DNA-L]

Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]

Solving, we get: [DNA-L] = [TotalDNA][L] / (k_off/k_on + L)

Usually, we define the dissociation constant to be: kD = k_off/k_on

So we end up with: [DNA-L] = [TotalDNA][L] / (kD + L)


Other oscillators

Repressilator

Did not end up working too well, but still ground-breaking for its time.

A ---| B ---| C ---| A

The original paper.

Repressilator on Wikipedia.

See also

Personal tools