# IGEM:Stanford/2010/Notebook/15 April 2010

(Difference between revisions)
 Revision as of 00:40, 16 April 2010 (view source) (→Journal Club 4/15/10)← Previous diff Revision as of 00:40, 16 April 2010 (view source) (→Repressilator)Next diff → Line 33: Line 33: The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper]. The [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html original paper]. - [[wikipedia:Repressilator]] on Wikipedia. + + [[wikipedia:Repressilator|Repressilator]] on Wikipedia. ===See also=== ===See also===

## Journal Club 4/15/10

Presentation by ...

### DNA-ligand binding

d[DNA-L]/dt = k_on*[DNA][L] - k_off*[DNA-L]

If we define: [TotalDNA] = [DNA] + [DNA-L]

Then: d[DNA-L]/dt = k_on*([TotalDNA] - [DNA-L]) - k_off*[DNA-L]

Solving, we get: [DNA-L] = [TotalDNA][L] / (k_off/k_on + L)

Usually, we define the dissociation constant to be: kD = k_off/k_on

So we end up with: [DNA-L] = [TotalDNA][L] / (kD + L)

### Other oscillators

#### Repressilator

Did not end up working too well, but still ground-breaking for its time.

A ---| B ---| C ---| A

The original paper.

Repressilator on Wikipedia.