IGEM:Stanford/2010/Notebook/mRNA sRNA

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General Ideas

One Idea to measure [B] / [A] (assume [A] ≠ 0)

  • input A induces expression of GFP mRNA
  • input B induces expression of GFP sRNA (sRNA that targets the GFP mRNA transcript)
  • so for a given [A],
    • if [B] = 0, we should see glowing bacteria
    • if 0 < [B] < [A], we should see some glow
    • if [B] > [A], we should see no glow

Another Idea to measure [B] / [A] (assume [A] ≠ 0)

  • input A induces expression of GFP mRNA and YFP sRNA
  • input B induces expression of YFP mRNA and GFP sRNA
  • so for a given [A],
    • if [B] = 0, we should see GREEN glowing bacteria
    • if 0 < [B] < [A], we should see some glow that is more GREEN than YELLOW.
    • if [B] > [A], we should see some glow that is more YELLOW than GREEN.

References

  • here's a good reference: Engineering RNA-based circuits. It discusses different methods of regulation by RNA:
    • via base-pairing (small RNA)
    • shape-based (riboswitches)
    • catalytic (ribozymes)
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