IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/07/12
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We decided to run an agarose 1% gel electrophoresis to confirm what we got on the samples | We decided to run an agarose 1% gel electrophoresis to confirm what we got on the samples | ||
| - | [[Image:Gel1207.jpg|thumb|center|]] | + | [[Image:Gel1207.jpg|thumb|center|upright=1.75|]] |
| + | Expected sizes : | ||
| + | 1.A = 4.6 kb | ||
| + | 1.B = 2.1 kb | ||
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| + | 1.C = 2.6 kb | ||
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| + | 1.D = 2.8 kb | ||
| + | |||
| + | 5.A = 2.1 kb | ||
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| + | 5.B =3.3 kb | ||
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| + | On this gel we can see that there is a purity problem on 1.C sample | ||
| + | |||
| + | and that 1.B samples are out of place , near the 3.5 kb marker | ||
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| + | tommorow we will run a gel after restriction to identify the problem with 1.B and 1.C samples | ||
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 Bacterial eyespot
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Day 8Every tube grown well overnight except for the two 1.E tubes wich are clear after centrifugation we confirmed that nothing grew on the two 1.E tubes We did a miniprep for the other tubes , We used our new GeneJET™ Plasmid Miniprep Kit [1] We checked the samples concentration with the nanodrop [2]  We got better results than with the QIAGEN® kit We decided to run an agarose 1% gel electrophoresis to confirm what we got on the samples  Expected sizes : 1.A = 4.6 kb 1.B = 2.1 kb 1.C = 2.6 kb 1.D = 2.8 kb 5.A = 2.1 kb 5.B =3.3 kb On this gel we can see that there is a purity problem on 1.C sample and that 1.B samples are out of place , near the 3.5 kb marker tommorow we will run a gel after restriction to identify the problem with 1.B and 1.C samples | |
