IGEM:The Citadel/Notebook/Construction

Biobrick construction map:

1: E0240 (A) -> J04450(C) = J23102 (C, ex A)
2: B0015 (A) -> J04450(C) = B0015 (C, ex A)
3: C0160 (A) -> J04450(C) = C0160 (C, ex A)
4: F1610 (A) -> J04450 (C) = F1610 (C, ex A)

5: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) [QS Stage 1]
6: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K)
7: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K)
8: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K)
9: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K)

10: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)
11: ~I50B15 (K) + I746360 (A) -> J04450(C) = K274370 (C)
12: ~I51B15 (K) + I746361 (A) -> J04450(C) = K274381 (C)
13: ~I52B15 (K) + I746365 (A) -> J04450(C) = K274395 (C)

14. F2620 (A) + K274382 (C) -> J04450(K) = ~F20K82 (K)

Biobrick Inventory:

1. J04450 (C) - 08/16 - 205.4 ng/ul 2. J04450 (K) - 08/16 - 227.1 ng/ul