# IGEM:The Citadel/Notebook/Construction

### From OpenWetWare

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Biobrick construction map: | Biobrick construction map: | ||

- | 1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated<br> | + | 1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated. Minipreped.<br> |

- | 2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated<br> | + | 2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated. Minipreped.<br> |

- | 3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated<br> | + | 3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated. Minipreped.<br> |

- | 4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K)<br> | + | 4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) -- transformed and plated.<br> |

- | 5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K)<br> | + | 5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K) -- transformed and plated.<br> |

- | 6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K)<br> | + | 6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K) -- transformed and plated.<br> |

- | 7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K)<br> | + | 7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K) -- transformed and plated.<br> |

- | 8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K)<br> | + | 8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K) -- transformed and plated.<br> |

9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)<br> | 9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)<br> | ||

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1. J04450 (C) - 08/18 - 355.9 ng/ul<br> | 1. J04450 (C) - 08/18 - 355.9 ng/ul<br> | ||

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+ | ==Notes== | ||

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+ | * I will provide information about expected band sizes on the gels that you guys run this weekend. -B | ||

+ | * Patrick, please update the "Biobrick Inventory" section as you miniprep each assembly component. Thanks! -B |

## Revision as of 11:11, 27 August 2010

Biobrick construction map:

1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated. Minipreped.

2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated. Minipreped.

3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated. Minipreped.

4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) -- transformed and plated.

5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K) -- transformed and plated.

6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K) -- transformed and plated.

7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K) -- transformed and plated.

8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K) -- transformed and plated.

9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)

10: ~I50B15 (K) + I746360 (A) -> J04450(C) = K274370 (C)

11: ~I51B15 (K) + I746361 (A) -> J04450(C) = K274381 (C)

12: ~I52B15 (K) + I746365 (A) -> J04450(C) = K274395 (C)

13. F2620 (A) + K274382 (C) -> J04450(K) = ~F20K82 (K)

14.

Biobrick Inventory:

1. J04450 (C) - 08/18 - 355.9 ng/ul

## Notes

- I will provide information about expected band sizes on the gels that you guys run this weekend. -B
- Patrick, please update the "Biobrick Inventory" section as you miniprep each assembly component. Thanks! -B