IGEM:The Citadel/Notebook/Construction: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
Biobrick construction map: | Biobrick construction map: | ||
1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated<br> | 1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated. Minipreped.<br> | ||
2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated<br> | 2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated. Minipreped.<br> | ||
3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated<br> | 3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated. Minipreped.<br> | ||
4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K)<br> | 4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) -- transformed and plated.<br> | ||
5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K)<br> | 5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K) -- transformed and plated.<br> | ||
6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K)<br> | 6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K) -- transformed and plated.<br> | ||
7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K)<br> | 7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K) -- transformed and plated.<br> | ||
8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K)<br> | 8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K) -- transformed and plated.<br> | ||
9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)<br> | 9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)<br> | ||
| Line 22: | Line 22: | ||
1. J04450 (C) - 08/18 - 355.9 ng/ul<br> | 1. J04450 (C) - 08/18 - 355.9 ng/ul<br> | ||
==Notes== | |||
* I will provide information about expected band sizes on the gels that you guys run this weekend. -B | |||
* Patrick, please update the "Biobrick Inventory" section as you miniprep each assembly component. Thanks! -B | |||
Revision as of 08:11, 27 August 2010
Biobrick construction map:
1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated. Minipreped.
2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated. Minipreped.
3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated. Minipreped.
4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) -- transformed and plated.
5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K) -- transformed and plated.
6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K) -- transformed and plated.
7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K) -- transformed and plated.
8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K) -- transformed and plated.
9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)
10: ~I50B15 (K) + I746360 (A) -> J04450(C) = K274370 (C)
11: ~I51B15 (K) + I746361 (A) -> J04450(C) = K274381 (C)
12: ~I52B15 (K) + I746365 (A) -> J04450(C) = K274395 (C)
13. F2620 (A) + K274382 (C) -> J04450(K) = ~F20K82 (K)
14.
Biobrick Inventory:
1. J04450 (C) - 08/18 - 355.9 ng/ul
Notes
- I will provide information about expected band sizes on the gels that you guys run this weekend. -B
- Patrick, please update the "Biobrick Inventory" section as you miniprep each assembly component. Thanks! -B
