IGEM:The Citadel/Notebook/Construction

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Biobrick construction map:

1: B0015 (A) -> J04450(C) = B0015 (C, ex A) -- transformed and plated. Minipreped.
2: C0160 (A) -> J04450(C) = C0160 (C, ex A) -- transformed and plated. Minipreped.
3: F1610 (A) -> J04450 (C) = F1610 (C, ex A) -- transformed and plated. Minipreped.

4: J23102 (A) + F1610 (C) -> J04450(K) = ~J02F10 (K) -- transformed and plated.
5: B0034 (A) + C0160 (C) -> J04450(K) = ~B34C60 (K) -- transformed and plated.
6: I746350 (A) + B0015 (C) -> J04450(K) = ~I50B15 (K) -- transformed and plated.
7: I746351 (A) + B0015 (C) -> J04450(K) = ~I51B15 (K) -- transformed and plated.
8: I746352 (A) + B0015 (C) -> J04450(K) = ~I52B15 (K) -- transformed and plated.

9: ~B34C60 (K) + B0015 (C) -> J04450(A) = K218026 (A)
10: ~I50B15 (K) + I746360 (A) -> J04450(C) = K274370 (C)
11: ~I51B15 (K) + I746361 (A) -> J04450(C) = K274381 (C)
12: ~I52B15 (K) + I746365 (A) -> J04450(C) = K274395 (C)

13. F2620 (A) + K274382 (C) -> J04450(K) = ~F20K82 (K)
14.


Biobrick Inventory:

1. J04450 (C) - 08/18 - 355.9 ng/ul


Notes

  • I will provide information about expected band sizes on the gels that you guys run this weekend. -B
  • Patrick, please update the "Biobrick Inventory" section as you miniprep each assembly component. Thanks! -B
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