IGEM:The Citadel/Notebook/Notes Brian/05-30-10: Difference between revisions
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====Procedure==== | ====Procedure==== | ||
#Autoclaved all flasks and tubes. | #Autoclaved all flasks and tubes.<br> The 250mL tubes were autoclaved with caps off, so to ensure sterility we've stored them on ethanol-soaked paper. | ||
#Prechilled the rotor at 4°C.<br> All tubes and pipets will be chilled tomorrow morning upon arrival. | |||
#Prechilled the rotor at 4°C. | |||
#Inoculated 5mL LB Miller medium and placed in incubator for overnight growth at 37°C with rotation. <br> Austin Che calls for LB Lennox to lessen salt content. We had only LB Miller at the time of inoculation and have attempted to use that; we'll post an update! For tomorrow's 450mL culture, we've prepared 500mL of LB Lennox. | #Inoculated 5mL LB Miller medium and placed in incubator for overnight growth at 37°C with rotation. <br> Austin Che calls for LB Lennox to lessen salt content. We had only LB Miller at the time of inoculation and have attempted to use that; we'll post an update! For tomorrow's 450mL culture, we've prepared 500mL of LB Lennox. | ||
Revision as of 14:42, 30 May 2010
May 30
This is a continuation of our work from 05-29-10.
Protocol for Electrocompetent Cells
Procedure
- Autoclaved all flasks and tubes.
The 250mL tubes were autoclaved with caps off, so to ensure sterility we've stored them on ethanol-soaked paper. - Prechilled the rotor at 4°C.
All tubes and pipets will be chilled tomorrow morning upon arrival. - Inoculated 5mL LB Miller medium and placed in incubator for overnight growth at 37°C with rotation.
Austin Che calls for LB Lennox to lessen salt content. We had only LB Miller at the time of inoculation and have attempted to use that; we'll post an update! For tomorrow's 450mL culture, we've prepared 500mL of LB Lennox.
