IGEM:The Citadel/Notebook/Notes Brian/06-28-10: Difference between revisions

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Plated all of our parts for isolation for 100 ml of cells
Plated all of our parts for isolation for 100 ml of cells


When Brian was here on Sunday June, 27, 2010 he throew out the 25% b/c there were no cells
When Brian was here on Sunday June, 27, 2010 he threw out the 25% b/c there were no cells


We placed the plates in the incubator at 4:15...we got sick
We placed the plates in the incubator at 4:15...we got sick
Line 21: Line 21:


Boiled the Agar to make sure it was good to go and then autoclaved it
Boiled the Agar to make sure it was good to go and then autoclaved it
Marvelled at how much damage Brian did with the bleach on the counter
Made 500 ml of Kan plates

Revision as of 13:23, 28 June 2010

Miniprep of ten of our 13 parts

BBa_J23102 
BBa_R0062 
BBa_F2620 
BBa_R0040 
BBa_R0063 
BBa_B0015 
BBa_C0160 
BBa_J45200 (we are not 100% confident that this colony was introduced to the correct part. Confirm sequence soon.) 
BBa_E0240 
BBa_F1610

Plated all of our parts for isolation for 100 ml of cells

When Brian was here on Sunday June, 27, 2010 he threw out the 25% b/c there were no cells

We placed the plates in the incubator at 4:15...we got sick

Listened to awesome music

Boiled the Agar to make sure it was good to go and then autoclaved it

Marvelled at how much damage Brian did with the bleach on the counter

Made 500 ml of Kan plates