IGEM:The Citadel/Notebook/Notes Brian/07-27-10: Difference between revisions
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Biobrick construction map: | Biobrick construction map: | ||
0.1 J23102 (A)[E,P] + pSB1C3 (PCR)[E,P] = J23102 (C, ex A)<br> | 0.1 J23102 (A)[E,P] + pSB1C3 (PCR)[E,P] = J23102 (C, ex A) -- COMPLETED! (Tube A)<br> | ||
0.2 B0015 (A)[E,P] + pSB1C3 (PCR)[E,P] = B0015 (C, ex A)<br> | 0.2 B0015 (A)[E,P] + pSB1C3 (PCR)[E,P] = B0015 (C, ex A) -- COMPLETED! (Tube B)<br> | ||
0.3 C0160 (A)[E,P] + pSB1C3 (PCR)[E,P] = C0160 (C, ex A)<br> | 0.3 C0160 (A)[E,P] + pSB1C3 (PCR)[E,P] = C0160 (C, ex A) -- COMPLETED! (Tube C)<br> | ||
0.4 J23102 (C, ex A)[E,S] + E0240 (A)[X,P] + pSB1T3 [E,P] = 3A Assembly Test<br> | 0.4 J23102 (C, ex A)[E,S] + E0240 (A)[X,P] + pSB1T3 [E,P] = ~J02E40 [3A Assembly Test]<br> | ||
1.0 J23102 (C, ex A)[E,S] + F1610 (A)[X,P] + | 1.0 J23102 (C, ex A)[E,S] + F1610 (A)[X,P] + pSB1T3 [E,P] = ~J02F10 (T)<br> | ||
* J23102 to be cut with E & P and inserted into pSB1C3 prior to assembly. | * J23102 to be cut with E & P and inserted into pSB1C3 prior to assembly. | ||
2.0 I746350 (A) + B0015 (C, ex A) + | 2.0 I746350 (A) + B0015 (C, ex A) + pSB1T3 = ~I50B15 (T)<br> | ||
* B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly. | * B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly. | ||
2.1 ~I50B15 (T) + I746360 (A) + | 2.1 ~I50B15 (T) + I746360 (A) + pSB1C3 = K274370 (C)<br> | ||
2.2 ~I50B15 (T) + I746361 (A) + | 2.2 ~I50B15 (T) + I746361 (A) + pSB1C3 = K274371 (C)<br> | ||
2.3 ~I50B15 (T) + I746364 (A) + | 2.3 ~I50B15 (T) + I746364 (A) + pSB1C3 = K274374 (C)<br> | ||
2.4 ~I50B15 (T) + I746365 (A) + | 2.4 ~I50B15 (T) + I746365 (A) + pSB1C3 = K274375 (C)<br> | ||
3.0 I746351 (A) + B0015 (C, ex A) + | 3.0 I746351 (A) + B0015 (C, ex A) + pSB1T3 = ~I51B15 (T)<br> | ||
* B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly. | * B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly. | ||
3.1 ~I51B15 (T) + I746360 (A) + | 3.1 ~I51B15 (T) + I746360 (A) + pSB1C3 = K274380 (C)<br> | ||
3.2 ~I51B15 (T) + I746361 (A) + | 3.2 ~I51B15 (T) + I746361 (A) + pSB1C3 = K274381 (C)<br> | ||
3.3 ~I51B15 (T) + I746362 (A) + | 3.3 ~I51B15 (T) + I746362 (A) + pSB1C3 = K274382 (C)<br> | ||
3.4 ~I51B15 (T) + I746364 (A) + | 3.4 ~I51B15 (T) + I746364 (A) + pSB1C3 = K274384 (C)<br> | ||
4.0 I746352 (A) + B0015 (C, ex A) + | 4.0 I746352 (A) + B0015 (C, ex A) + pSB1T3 = ~I52B15 (T)<br> | ||
* B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly. | * B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly. | ||
4.1 ~I52B15 (T) + I746361 (A) + | 4.1 ~I52B15 (T) + I746361 (A) + pSB1C3 = K274391 (C)<br> | ||
4.2 ~I52B15 (T) + I746362 (A) + | 4.2 ~I52B15 (T) + I746362 (A) + pSB1C3 = K274392 (C)<br> | ||
4.3 ~I52B15 (T) + I746364 (A) + | 4.3 ~I52B15 (T) + I746364 (A) + pSB1C3 = K274394 (C)<br> | ||
4.4 ~I52B15 (T) + I746365 (A) + | 4.4 ~I52B15 (T) + I746365 (A) + pSB1C3 = K274395 (C)<br> | ||
5.0 F2620 (A) + K274382 (C) + | 5.0 F2620 (A) + K274382 (C) + pSB1T3 = ~F20K82 (T)<br> | ||
5.1 ~F20K82 (T) + E0240 (A) + | 5.1 ~F20K82 (T) + E0240 (A) + pSB1C3 = ~F20K82E40 (C)<br> | ||
5.2 ~J02F10 (T) + ~F20K82E40 (C) + | 5.2 ~J02F10 (T) + ~F20K82E40 (C) + pSB1A3 = ~J02F10F20K82E40 (A) [QS & ST tester]<br> | ||
6.0 B0034 (A) + C0160 (C, ex A) + | 6.0 B0034 (A) + C0160 (C, ex A) + pSB1T3 = ~B34C60 (T)<br> | ||
7.0 ~B34C60 (T) + B0015 (C, ex A) + | 7.0 ~B34C60 (T) + B0015 (C, ex A) + pSB1A3 = K218026 (A)<br> | ||
8.0 ~J02F10 (T) + F2620 (A) + pSB1C3 = ~J02F10F20 (C)<br> | |||
8.1 ~J02F10F20 (C) + E0240 (A) + pSB1T3 = ~J02F10F20E40 (T) [QS Test]<br> | |||
Latest revision as of 07:31, 28 July 2010
Biobrick construction map:
0.1 J23102 (A)[E,P] + pSB1C3 (PCR)[E,P] = J23102 (C, ex A) -- COMPLETED! (Tube A)
0.2 B0015 (A)[E,P] + pSB1C3 (PCR)[E,P] = B0015 (C, ex A) -- COMPLETED! (Tube B)
0.3 C0160 (A)[E,P] + pSB1C3 (PCR)[E,P] = C0160 (C, ex A) -- COMPLETED! (Tube C)
0.4 J23102 (C, ex A)[E,S] + E0240 (A)[X,P] + pSB1T3 [E,P] = ~J02E40 [3A Assembly Test]
1.0 J23102 (C, ex A)[E,S] + F1610 (A)[X,P] + pSB1T3 [E,P] = ~J02F10 (T)
- J23102 to be cut with E & P and inserted into pSB1C3 prior to assembly.
2.0 I746350 (A) + B0015 (C, ex A) + pSB1T3 = ~I50B15 (T)
- B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.
2.1 ~I50B15 (T) + I746360 (A) + pSB1C3 = K274370 (C)
2.2 ~I50B15 (T) + I746361 (A) + pSB1C3 = K274371 (C)
2.3 ~I50B15 (T) + I746364 (A) + pSB1C3 = K274374 (C)
2.4 ~I50B15 (T) + I746365 (A) + pSB1C3 = K274375 (C)
3.0 I746351 (A) + B0015 (C, ex A) + pSB1T3 = ~I51B15 (T)
- B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.
3.1 ~I51B15 (T) + I746360 (A) + pSB1C3 = K274380 (C)
3.2 ~I51B15 (T) + I746361 (A) + pSB1C3 = K274381 (C)
3.3 ~I51B15 (T) + I746362 (A) + pSB1C3 = K274382 (C)
3.4 ~I51B15 (T) + I746364 (A) + pSB1C3 = K274384 (C)
4.0 I746352 (A) + B0015 (C, ex A) + pSB1T3 = ~I52B15 (T)
- B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.
4.1 ~I52B15 (T) + I746361 (A) + pSB1C3 = K274391 (C)
4.2 ~I52B15 (T) + I746362 (A) + pSB1C3 = K274392 (C)
4.3 ~I52B15 (T) + I746364 (A) + pSB1C3 = K274394 (C)
4.4 ~I52B15 (T) + I746365 (A) + pSB1C3 = K274395 (C)
5.0 F2620 (A) + K274382 (C) + pSB1T3 = ~F20K82 (T)
5.1 ~F20K82 (T) + E0240 (A) + pSB1C3 = ~F20K82E40 (C)
5.2 ~J02F10 (T) + ~F20K82E40 (C) + pSB1A3 = ~J02F10F20K82E40 (A) [QS & ST tester]
6.0 B0034 (A) + C0160 (C, ex A) + pSB1T3 = ~B34C60 (T)
7.0 ~B34C60 (T) + B0015 (C, ex A) + pSB1A3 = K218026 (A)
8.0 ~J02F10 (T) + F2620 (A) + pSB1C3 = ~J02F10F20 (C)
8.1 ~J02F10F20 (C) + E0240 (A) + pSB1T3 = ~J02F10F20E40 (T) [QS Test]
