IGEM:The Citadel/Notebook/Notes Brian/07-27-10

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Biobrick construction map:

0.1 J23102 (A)[E,P] + pSB1C3 (PCR)[E,P] = J23102 (C, ex A) -- COMPLETED! (Tube A)
0.2 B0015 (A)[E,P] + pSB1C3 (PCR)[E,P] = B0015 (C, ex A) -- COMPLETED! (Tube B)
0.3 C0160 (A)[E,P] + pSB1C3 (PCR)[E,P] = C0160 (C, ex A) -- COMPLETED! (Tube C)
0.4 J23102 (C, ex A)[E,S] + E0240 (A)[X,P] + pSB1T3 [E,P] = 3A Assembly Test
1.0 J23102 (C, ex A)[E,S] + F1610 (A)[X,P] + pSB1T3 [E,P] = ~J02F10 (T)

  • J23102 to be cut with E & P and inserted into pSB1C3 prior to assembly.

2.0 I746350 (A) + B0015 (C, ex A) + pSB1T3 = ~I50B15 (T)

  • B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.

2.1 ~I50B15 (T) + I746360 (A) + pSB1C3 = K274370 (C)
2.2 ~I50B15 (T) + I746361 (A) + pSB1C3 = K274371 (C)
2.3 ~I50B15 (T) + I746364 (A) + pSB1C3 = K274374 (C)
2.4 ~I50B15 (T) + I746365 (A) + pSB1C3 = K274375 (C)
3.0 I746351 (A) + B0015 (C, ex A) + pSB1T3 = ~I51B15 (T)

  • B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.

3.1 ~I51B15 (T) + I746360 (A) + pSB1C3 = K274380 (C)
3.2 ~I51B15 (T) + I746361 (A) + pSB1C3 = K274381 (C)
3.3 ~I51B15 (T) + I746362 (A) + pSB1C3 = K274382 (C)
3.4 ~I51B15 (T) + I746364 (A) + pSB1C3 = K274384 (C)
4.0 I746352 (A) + B0015 (C, ex A) + pSB1T3 = ~I52B15 (T)

  • B0015 to be cut with E & P and inserted into pSB1C3 prior to assembly.

4.1 ~I52B15 (T) + I746361 (A) + pSB1C3 = K274391 (C)
4.2 ~I52B15 (T) + I746362 (A) + pSB1C3 = K274392 (C)
4.3 ~I52B15 (T) + I746364 (A) + pSB1C3 = K274394 (C)
4.4 ~I52B15 (T) + I746365 (A) + pSB1C3 = K274395 (C)
5.0 F2620 (A) + K274382 (C) + pSB1T3 = ~F20K82 (T)
5.1 ~F20K82 (T) + E0240 (A) + pSB1C3 = ~F20K82E40 (C)
5.2 ~J02F10 (T) + ~F20K82E40 (C) + pSB1A3 = ~J02F10F20K82E40 (A) [QS & ST tester]
6.0 B0034 (A) + C0160 (C, ex A) + pSB1T3 = ~B34C60 (T)
7.0 ~B34C60 (T) + B0015 (C, ex A) + pSB1A3 = K218026 (A)
8.0 ~J02F10 (T) + F2620 (A) + pSB1C3 = ~J02F10F20 (C)
8.1 ~J02F10F20 (C) + E0240 (A) + pSB1T3 = ~J02F10F20E40 [QS Test]

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