IGEM:The Citadel/Training: Difference between revisions

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If we want to do something in training that will really ''help iGEM and be credited towards our project'' we should consider requesting Biobrick promoters that previous teams have made but that have not yet received adequate testing / characterization.  By measuring these parts against a standard, we would get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer.  Plus, we could submit our results to the Registry and have already contributed before the summer even starts.
If we want to do something in training that will really ''help iGEM and be credited towards our project'' we should consider requesting Biobrick promoters that previous teams have made but that have not yet received adequate testing / characterization.  By measuring these parts against a standard, we would get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer.  Plus, we could submit our results to the Registry and have already contributed before the summer even starts.


* Everyone would need to be familiar with the [http://www.jbioleng.org/content/3/1/4 Kelly et al. paper] in order to do this training.  The supplementary materials are also important and can be safely downloaded by clicking [http://www.jbioleng.org/content/supplementary/1754-1611-3-4-s1.doc| here].  For a graphical summary of the protocol we will follow, travel over to .  (The images are also in the supplementary materials.)   
* Everyone would need to be familiar with the [http://www.jbioleng.org/content/3/1/4 Kelly et al. paper] in order to do this training.  The supplementary materials are also important and can be safely downloaded by clicking [http://www.jbioleng.org/content/supplementary/1754-1611-3-4-s1.doc| here].  For a graphical summary of the protocol we will follow, travel over to the [http://partsregistry.org/Measurement/SPU/Measure Parts Registry Measurement Section].  (The images are also in the supplementary materials.)   
** Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter).  Instead, we will be testing promoters that have already been made by previous iGEM teams.  This will save us money and it helps out iGEM tremendously to have this QA performed.
** Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter).  Instead, we will be testing promoters that have already been made by previous iGEM teams.  This will save us money and it helps out iGEM tremendously to have this QA performed.



Revision as of 01:34, 9 April 2010

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OPTION ONE

If we want to do something in training that will really help iGEM and be credited towards our project we should consider requesting Biobrick promoters that previous teams have made but that have not yet received adequate testing / characterization. By measuring these parts against a standard, we would get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer. Plus, we could submit our results to the Registry and have already contributed before the summer even starts.

  • Everyone would need to be familiar with the Kelly et al. paper in order to do this training. The supplementary materials are also important and can be safely downloaded by clicking here. For a graphical summary of the protocol we will follow, travel over to the Parts Registry Measurement Section. (The images are also in the supplementary materials.)
    • Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter). Instead, we will be testing promoters that have already been made by previous iGEM teams. This will save us money and it helps out iGEM tremendously to have this QA performed.

OPTION TWO

If we wanted to work directly in our area(s) of interest for summer research, we could ask for a Distribution Kit and begin running preliminary assembly experiments. Though it may be to early/late to get a complete distribution.

OPTION THREE

If we want to do something generic in order to have the most basic level of comprehension, we could opt to perform the transformations outlined in The ETecher's Guide to Biological Engineering. The procedures are simple and we could easily execute them while at the same time getting a lot out experience for our effort.

We have a few different approaches to how we want to begin investigating the iGEM process. Take a look at the above options and voice your opinion about how we should proceed.