IGEM:The Citadel/Training: Difference between revisions

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==OPTION ONE==
==May Crash Course in Synthetic Biology==
If we want to do something in training that will really ''help iGEM and be credited towards our project'' we should consider requesting Biobrick promoters that previous teams have made but that have not yet received adequate testing / characterization.  By measuring these parts against a standard, we would get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer.  Plus, we could submit our results to the Registry and have already contributed before the summer even starts.


* Everyone would need to be familiar with the [http://www.jbioleng.org/content/3/1/4 Kelly et al. paper] in order to do this training.  The supplementary materials are also important and can be safely downloaded by clicking [http://www.jbioleng.org/content/supplementary/1754-1611-3-4-s1.doc| here].  For a graphical summary of the protocol we will follow, travel over to the [http://partsregistry.org/Measurement/SPU/Measure Parts Registry Measurement Section].  (The images are also in the supplementary materials.) 
In order to get some serious training uner our belt, do something that ''helps iGEM, and start work that can be credited towards our project'' we will be testing Biobrick promoter strength on May 6-10th.
** Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter).  Instead, we will be testing promoters that have already been made by previous iGEM teams.  This will save us money and it helps out iGEM tremendously to have this QA performed.


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==OPTION TWO==
* Request Biobrick promoters that previous teams have made but that have not yet received adequate characterization. (To be done NTL April 25)
If we wanted to work directly in our area(s) of interest for summer research, we could ask for a Distribution Kit and begin running preliminary assembly experiments. Though it may be too early/late to get a complete distribution.  
* Perform assembly of promoters into plasmid vectors.
* Transform ''E.coli'' with the constructed plasmids and grow up colonies.
* Measure GFP expression of test promoter against reference standard.


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==OPTION THREE==
To do this training, make sure that you are familiar with [http://www.jbioleng.org/content/3/1/4 "Measuring the activity of Biobrick Promoters Using an ''In vivo'' Reference Standard"] by ''Jason Kelly et al.''.  The supplementary materials are also important and can be safely downloaded by clicking on[http://www.jbioleng.org/content/supplementary/1754-1611-3-4-s1.doc| this link here].  For a graphical summary of the protocol, travel over to the [http://partsregistry.org/Measurement/SPU/Measure Parts Registry Measurement Section].  (The images are also in the supplementary materials.) 
If we want to do something generic in order to have the most basic level of comprehension, we could opt to perform the transformations outlined in ''The ETecher's Guide to Biological Engineering''.  The procedures are simple and we could easily execute them while at the same time getting a lot out experience for our effort.


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We have a few different approaches to how we want to begin investigating the iGEM process.  Take a look at the above options and voice your opinion about how we should proceed.
By measuring these parts against a standard, we'll get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer.  Plus, we could submit our results to the Registry and have already contributed before the summer even starts.
 
 
* Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter).  Instead, we will be testing promoters that have already been made by previous iGEM teams.  This will save us money and it helps iGEM out to have this QA performed.

Revision as of 13:01, 19 April 2010

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May Crash Course in Synthetic Biology

In order to get some serious training uner our belt, do something that helps iGEM, and start work that can be credited towards our project we will be testing Biobrick promoter strength on May 6-10th.


  • Request Biobrick promoters that previous teams have made but that have not yet received adequate characterization. (To be done NTL April 25)
  • Perform assembly of promoters into plasmid vectors.
  • Transform E.coli with the constructed plasmids and grow up colonies.
  • Measure GFP expression of test promoter against reference standard.


To do this training, make sure that you are familiar with "Measuring the activity of Biobrick Promoters Using an In vivo Reference Standard" by Jason Kelly et al.. The supplementary materials are also important and can be safely downloaded by clicking onthis link here. For a graphical summary of the protocol, travel over to the Parts Registry Measurement Section. (The images are also in the supplementary materials.)


By measuring these parts against a standard, we'll get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer. Plus, we could submit our results to the Registry and have already contributed before the summer even starts.


  • Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter). Instead, we will be testing promoters that have already been made by previous iGEM teams. This will save us money and it helps iGEM out to have this QA performed.
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