IGEM:The Citadel/Training
OPTION ONE
If we want to do something in training that will really help iGEM and be credited towards our project we should consider requesting Biobrick promoters that previous teams have made but that haven't been well tested. By measuring these parts against a standard, we would get a tremendous amount of experience in nearly every protocol we need for the summer. Plus, we could submit our results to the Registry and have already contributed before the summer even starts.
- You will need to be familiar with this paper in order to do the training. The supplementary materials are also important and can be safely downloaded by clicking here. For a graphical summary of the protocol we will follow, see the images below. (The images are also in the supplementary materials.)
- Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter). Instead, we will be testing promoters that have already been made by previous iGEM teams. This will save us money and it helps out iGEM tremendously.
[Promoter Measuring Protocol Sheet One]
[Promoter Measuring Protocol Sheet Two]
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OPTION TWO
If we wanted to work directly in our area(s) of interest for summer research, we could ask for a Distribution Kit and begin running preliminary assembly experiments.
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OPTION THREE
If we want to do something generic in order to have the most basic level of comprehension, we could opt to perform the transformations outlined in The ETech Guide to Biological Engineering. The procedures are simple and we could easily at the same time getting a lot out of them.
