May Crash Course in Synthetic Biology
In order to get some serious training uner our belt, do something that helps iGEM, and start work that can be credited towards our project we will be testing Biobrick promoter strength on May 6-10th.
- Request Biobrick promoters that previous teams have made but that have not yet received adequate characterization. (To be done NTL April 25)
- Perform assembly of promoters into plasmid vectors.
- Transform E.coli with the constructed plasmids and grow up colonies.
- Measure GFP expression of test promoter against reference standard.
To do this training, make sure that you are familiar with "Measuring the activity of Biobrick Promoters Using an In vivo Reference Standard" by Jason Kelly et al.. The supplementary materials are also important and can be safely downloaded by clicking onthis link here. For a graphical summary of the protocol, travel over to the Parts Registry Measurement Section. (The images are also in the supplementary materials.)
By measuring these parts against a standard, we'll get a tremendous amount of experience in many of the protocols we'll need to have mastered for summer. Plus, we could submit our results to the Registry and have already contributed before the summer even starts.
- Please note that we WILL NOT be doing Step #1 (synthesis and annealing of oligos to make a test promoter). Instead, we will be testing promoters that have already been made by previous iGEM teams. This will save us money and it helps iGEM out to have this QA performed.
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