IGEM:Tsinghua/2007/Projects/Celcuit

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<h1>Flowchart</h1>
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<h1>Celcuit</h1>
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<h2>Introduction</h2>
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<table width="100%">
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<tr><td>
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<h3>Standards in engineering cell-cell communications.</h3></td></tr>
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<tr><td>
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In generally, when engineering prokaryotic cell networks, one of the most
 +
difficulties is to isolate signals. On one hand, in the widely-used
 +
quorum-sensing system, the quorum-sensing signal is excreted out of the cells,
 +
which in spite the concentration gradient in space broadcasts an identical signal to
 +
every recipient cell. Therefore, cells with same receptors will be triggered
 +
simultaneously. And when trying to engineering a more complex system, the
 +
efforts are generally limited by available kinds of signal producers and
 +
receptors. On the other hand, space isolation is equally important in
 +
engineering. Take the isolated wire cable as an example, although all the wires
 +
utilized identical electric current as the medium for signal transport, the isolation
 +
allows identical electric current in different wires to generate various combinations of
 +
signals. While the excreted broadcasting prokaryotic signals are unable to work in this
 +
way.
 +
Therefore, those requirements bring the problem posed in the field of
 +
bioengineering into a general topic in engineering cell-cell communications.
 +
It's necessary to establish a system with standards in cell-cell communication with expected isolation and variance in the kinds of signals.</td></tr>
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<tr><td>
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<h3> </h3></td></tr>
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<tr><td>
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 </td></tr>
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</table>
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<h2>Strains, Plasmids & Materials</h2>
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<h2>Protocols</h2>
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<h2>Flowchart</h2>
[[Image:CelCuitv1.5.gif]]
[[Image:CelCuitv1.5.gif]]
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<h2>Eperiment Records</h2>
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<h3>Thread 1: Construction of the Celcuit carrier plasmids.</h3>
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Nubmer</td>
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Date</td>
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Inherit</td>
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ChartIndex</td>
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Notes</td>
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Results</td>
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Plan</td>
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Expected Time</td>
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Oprator</td>
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 </td>
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 </td>
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</table>

Revision as of 09:48, 2 August 2007

Contents

Celcuit

Introduction

Standards in engineering cell-cell communications.

In generally, when engineering prokaryotic cell networks, one of the most difficulties is to isolate signals. On one hand, in the widely-used quorum-sensing system, the quorum-sensing signal is excreted out of the cells, which in spite the concentration gradient in space broadcasts an identical signal to every recipient cell. Therefore, cells with same receptors will be triggered simultaneously. And when trying to engineering a more complex system, the efforts are generally limited by available kinds of signal producers and receptors. On the other hand, space isolation is equally important in engineering. Take the isolated wire cable as an example, although all the wires utilized identical electric current as the medium for signal transport, the isolation allows identical electric current in different wires to generate various combinations of signals. While the excreted broadcasting prokaryotic signals are unable to work in this way. Therefore, those requirements bring the problem posed in the field of bioengineering into a general topic in engineering cell-cell communications.

It's necessary to establish a system with standards in cell-cell communication with expected isolation and variance in the kinds of signals.

 

 

Strains, Plasmids & Materials

Protocols

Flowchart

Image:CelCuitv1.5.gif


Eperiment Records

Thread 1: Construction of the Celcuit carrier plasmids.

Nubmer Date Inherit ChartIndex Notes Results Plan Expected Time Oprator
                 
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