IGEM:UC-Merced/2009/Notebook/E. hydro Express

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Description/Abstract

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Notes

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Example: Next team meeting on Tuesday, June 18th! Be there!

Lab Notebook

Beginning Tuesday November 13, 2012

10/13/2012

 Phage

  1. Prepared Stocks
   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12

10/14/12

 Phage

1. Overnight culture did not grow. 2. Prepared new stock with frozen bacteria (procedure carried out as on 10/13/12)

   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12

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