IGEM:UC-Merced/2009/Notebook/E. hydro Express
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Description/Abstract
Notes
Lab Notebook Beginning Tuesday November 13, 2012 10/13/2012 Generation P1 Phage Stock 1. Prepared P1 Stock from Yale University
a. LB
b. Kanamycin stock 25mg/ml to add to strain JW1228-1
C1V1=C2V2
25000 ug/ml (V1) = 50ug/ml (5ml)
C1 = 25000 ug/ml --> kanamycin concentration
C2 = 50 ug/ml
V2 = 5 ml broth
25000 ug/ml (V1) = 250 ug
V1 = 0.01 ml = 10 uL --> kanamycin to add to strain JW1228-1
Incubate kanomycin in LB broth until 11am 10/14/12
P1 Phage 1. Overnight culture did not grow. Possible reasons are: (1) The JW1228-1 culture used was old and found in the 4°C refrigerator. It is possible that the culture was no longer living. To address this possibility we will redo the procedure using frozen stock. 2. Prepared new overnight culture with frozen JW1228-1 glycerol stock (procedure carried out as on 10/13/12) a. LB
b. Kanamycin stock 25mg/ml to add to strain JW1228-1
C1V1=C2V2
25000 ug/ml (V1) = 50ug/ml (5ml)
C1 = 25000 ug/ml --> kanamycin concentration
C2 = 50 ug/ml
V2 = 5 ml broth
25000 ug/ml (V1) = 250 ug
V1 = 0.01 ml = 10 uL --> kanamycin to add to strain JW1228-1
Incubate kanomycin in LB broth until 10 am 10/15/12
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Recent changes 19 May 2013
16 May 2013
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17:48
