IGEM:UC-Merced/2009/Notebook/E. hydro Express

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Beginning Tuesday November 13, 2012
Beginning Tuesday November 13, 2012
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10/13/2012
   Phage
   Phage
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       25000 ug (V1) - 250 ug (ml)
       25000 ug (V1) - 250 ug (ml)
       V1 = 0.01 ml = 10 uL  --> kanomycin to add to strain JW1228-1  
       V1 = 0.01 ml = 10 uL  --> kanomycin to add to strain JW1228-1  
 +
      Incubate kanomycin in LB broth until 11am 10/14/12
 +

Revision as of 21:37, 13 November 2012

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Lab Notebook

Beginning Tuesday November 13, 2012

10/13/2012 

 Phage

  1. Prepared Stocks
   a. LB
   b. Kanomycin to add to strain JW1228-1
       C1V1=C2V2
      25000 ug (V1) = 50ug (5ml)
      C1 = 25000 ug  --> kanomycin concentration
      C2 = 50 ug,   V2 = 5 ml broth
      25000 ug (V1) - 250 ug (ml)
      V1 = 0.01 ml = 10 uL   --> kanomycin to add to strain JW1228-1 
     Incubate kanomycin in LB broth until 11am 10/14/12






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