IGEM:UC/2006-6-7: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
JCAnderson (talk | contribs) No edit summary |
JCAnderson (talk | contribs) No edit summary |
||
| Line 5: | Line 5: | ||
#15ug/mL gentamicin (G) | #15ug/mL gentamicin (G) | ||
#50ug/mL ampicillin (A) | #50ug/mL ampicillin (A) | ||
#25ug/mL chloramphenical (C) | #25ug/mL chloramphenical (C) ---errr, axe that datapoint, the plate disapeared.--[[User:JCAnderson|JCAnderson]] 13:45, 8 June 2006 (EDT) | ||
#50ug/mL spectinamycin (Sp) | #50ug/mL spectinamycin (Sp) | ||
#25ug/mL tetracycline (Tet) | #25ug/mL tetracycline (Tet) | ||
Latest revision as of 10:45, 8 June 2006
JL_06/07/06
Goal: We want to determine the antibiotics resistence of the F and R knockout plasmids (TlambdaOlambda)
Results:
We grew TlambdaOlambdaRlambda to early log density. We then streaked the cultures with pipette tips on LB agar plates containing the following anti-biotics:
- 15ug/mL gentamicin (G)
- 50ug/mL ampicillin (A)
- 25ug/mL chloramphenical (C) ---errr, axe that datapoint, the plate disapeared.--JCAnderson 13:45, 8 June 2006 (EDT)
- 50ug/mL spectinamycin (Sp)
- 25ug/mL tetracycline (Tet)
- 20ug/mL trimethylprim (Trim)
TlambdaOlambda grerw on kan only
Rlambda grew on kan and tet only--JCAnderson 19:07, 7 June 2006 (EDT)
