IGEM:UC Berkeley/2006

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 111: Line 111:
'''Subgroup Strategies, Overview'''
'''Subgroup Strategies, Overview'''
-
*[[logic gate overview]]
+
*[[Powerpoint presentations, drafts]]
|}
|}

Revision as of 02:00, 29 August 2006


UC Berkeley

We are researching addressable cell-to-cell communication in E. coli. By manipulating the process of conjugation, our cells may selectively pass plasmids to other cells. Information contained in the transferred plasmids may be regulated translationally by implementing RNA based locks and keys. These key research advances may allow for implementation of complex systems of NAND logic gates in bacterial cultures.
Image:GettingStarted iconbaby.png Getting Started on OWW


Resources Team


Undergrads

Bryan Hernandez
Matt Fleming
Kaitlin A. Davis
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth

Postdocs

John Dueber
Chris Anderson

News Tools


lab calendar

The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Procedure Overview (Plasmid DNA to Sequencing)
Plasmid Transformation
Digestion
Ligation
Sequencing
Competent cell production
PCR prep
Conjugation
DNA Gel pouring
DNA purifications
PCR machine program (Expand kit)
Knockouts
Knockouts by Electroporation of pOX38 x pKD46


Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook


Resources Project


Addressable Cell-to-Cell Communication

Useful Links


Subgroup Strategies, Overview

Personal tools