IGEM:UC Berkeley/2006

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| rowspan=2 | [[Image:Berkeley.jpg|left|200px]]'''A Bacterial model for Neural Learning'''
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| rowspan=2 | [[Image:Berkeley.jpg|left|200px]]'''Addressible Conjugation'''
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Information is assimilated in neural systems by an architecture of cell-based switches interconnected by communication channels.  These channels are based on physical, spatial connections between cells in the form of dendrite and axons. We are constructing a bacterial model of neural systems where individual bacteria take the place of neurons and communicate with one another through addressable conjugation. Here, bacteria can send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based locks and keys. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to explore different architectures of neural systems to better understand the mechanisms of learning.  <br>   
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Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.  <br>   
[[Image:GettingStarted iconbaby.png]] [[OpenWetWare:Getting started|'''Getting Started on OWW''']]<br>
[[Image:GettingStarted iconbaby.png]] [[OpenWetWare:Getting started|'''Getting Started on OWW''']]<br>
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Revision as of 20:39, 27 October 2006


Addressible Conjugation

Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.
Image:GettingStarted iconbaby.png Getting Started on OWW


Resources Team


Undergrads

Bryan Hernandez
Matt Fleming
Kaitlin A. Davis
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth

Postdocs

John Dueber
Chris Anderson

News Tools


lab calendar

The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Procedure Overview (Plasmid DNA to Sequencing)
Plasmid Transformation
Digestion
Ligation
Sequencing
Competent cell production
PCR prep
Conjugation
DNA Gel pouring
DNA purifications
PCR machine program (Expand kit)
Knockouts
Knockouts by Electroporation of pOX38 x pKD46


Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - 09/07/06
Conjugation 09/07/06- present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook


Resources Project


Addressable Cell-to-Cell Communication

Useful Links


Subgroup Strategies, Overview

Personal tools