IGEM:UC Berkeley/2006: Difference between revisions
JCAnderson (talk | contribs) No edit summary |
JCAnderson (talk | contribs) No edit summary |
||
Line 63: | Line 63: | ||
[[IGEM:UC Berkeley/2006/Sequencing|Sequencing]]<br> | [[IGEM:UC Berkeley/2006/Sequencing|Sequencing]]<br> | ||
[[IGEM:UC Berkeley/2006/Competent cell production|Competent cell production]]<br> | [[IGEM:UC Berkeley/2006/Competent cell production|Competent cell production]]<br> | ||
[[IGEM:UC Berkeley/2006/OverlapExtensionRxn|Overlap extension of oligos]]<br> | |||
[[IGEM:UC Berkeley/2006/PCRPrep|PCR prep]]<br> | [[IGEM:UC Berkeley/2006/PCRPrep|PCR prep]]<br> | ||
[[IGEM:UC Berkeley/2006/Conjugation|Conjugation]]<br> | [[IGEM:UC Berkeley/2006/Conjugation|Conjugation]]<br> |
Revision as of 15:03, 15 November 2006
Addressable Conjugation in Bacterial Networks
Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning. |