IGEM:UC Berkeley/2006

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[[IGEM:UC Berkeley/2006/Sequencing|Sequencing]]<br>
[[IGEM:UC Berkeley/2006/Sequencing|Sequencing]]<br>
[[IGEM:UC Berkeley/2006/Competent cell production|Competent cell production]]<br>
[[IGEM:UC Berkeley/2006/Competent cell production|Competent cell production]]<br>
[[IGEM:UC Berkeley/2006/OverlapExtensionRxn|Overlap extension of oligos]]<br>
[[IGEM:UC Berkeley/2006/PCRPrep|PCR prep]]<br>
[[IGEM:UC Berkeley/2006/PCRPrep|PCR prep]]<br>
[[IGEM:UC Berkeley/2006/Conjugation|Conjugation]]<br>
[[IGEM:UC Berkeley/2006/Conjugation|Conjugation]]<br>

Revision as of 18:03, 15 November 2006

Addressable Conjugation in Bacterial Networks

Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.
The presentation of our results for the 2006 Jamboree is available at the iGEM Website

Resources Team


Bryan Hernandez
Matt Fleming
Kaitlin A. Davis
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth


John Dueber
Chris Anderson

News Tools

lab calendar

The Details

Construction Files
-80 Stocks


Procedure Overview (Plasmid DNA to Sequencing)
Plasmid Transformation
Competent cell production
Overlap extension of oligos
PCR prep
DNA Gel pouring
DNA purifications
PCR machine program (Expand kit)
Knockouts by Electroporation of pOX38 x pKD46

Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - 09/07/06
Conjugation 09/07/06- present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook

Resources Project

Addressable Cell-to-Cell Communication

Useful Links

Subgroup Strategies, Overview

Personal tools