IGEM:UC Berkeley/2006

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UC Berkeley

We plan to create an addressable cell-to-cell communication mechanism in e.coli. Communication will be achieved through the selective swapping of two plasmids, one from each cell. When conjugation occurs a promoter will be turned on in the receiptient cell and it will express GFP thereby illuminating it and signaling that communication was successful...someone who understands this well should really write something better here.
Image:GettingStarted iconbaby.png Getting Started on OWW


Resources Team


Undergrads

Bryan Hernandez
Matt Fleming
Kaitlin A. Davis
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth

Postdocs

John Dueber
Chris Anderson

News Tools


lab calendar

The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Procedure Overview (Plasmid DNA to Sequencing)
Plasmid Transformation
Digestion
Ligation
Sequencing
Competent cell production
PCR prep
Conjugation
DNA Gel pouring
Short DNA frag cleanup
DNA purifications
PCR machine program (extend kit)
Knockouts
PCR Cleanup

Subgroup Notebooks

Conjugation
Locks and Keys
Logic Gates

Resources Project


Addressable Cell-to-Cell Communication

Useful Links

Personal tools