| UC Berkeley |
We plan to create an addressable cell-to-cell communication mechanism in e.coli. Communication will be achieved through the selective swapping of two plasmids, one from each cell. When conjugation occurs a promoter will be turned on in the receiptient cell and it will express GFP thereby illuminating it and signaling that communication was successful...someone who understands this well should really write something better here.
Procedure Overview (Plasmid DNA to Sequencing)
Addressable Cell-to-Cell Communication