IGEM:UC Berkeley/2006/Conjugation
From OpenWetWare
Rlambda (marker swap Procedure)
TlambdaOlambda
protocol for conjugation
donor protocol
- start out with saturated 1 mL of culture
- spin down (top speed for 10 sec.) , discard fluid
- resuspend in 1 mL LB
- vortex, spin, discard fluid
- resuspend in 1 mL LB, vortex
- put 200uL of culture into 2 mL of LB (if arabinose is needed put in 100 mg/mL)
- grow 2 hours
- do Assay
Recipient protocol
- take aliquot and thaw
- dilute 10 fold into LB (ie. put 200 uL into 2 mL of LB)
- grow 2 hours
- do Assay