IGEM:UC Berkeley/2006/Conjugation

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Rlambda (marker swap Procedure)
TlambdaOlambda

protocol for conjugation

donor protocol

  1. start out with saturated 1 mL of culture
  2. spin down (top speed for 10 sec.) , discard fluid
  3. resuspend in 1 mL LB
  4. vortex, spin, discard fluid
  5. resuspend in 1 mL LB, vortex
  6. put 200uL of culture into 2 mL of LB (if arabinose is needed put in 100 mg/mL)
  7. grow 2 hours
  8. do Assay

Recipient protocol

  1. take aliquot and thaw
  2. dilute 10 fold into LB (ie. put 200 uL into 2 mL of LB)
  3. grow 2 hours
  4. do Assay
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