IGEM:UC Berkeley/2006/Ligation

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Line 6: Line 6:
   * 2 uL insert <br>
   * 2 uL insert <br>
 +
2. Let ligate at room temp for 30 minutes <br>
  Note: For the control, add 2 uL of water instead of the insert
  Note: For the control, add 2 uL of water instead of the insert

Revision as of 18:04, 19 June 2006

1. Create Ligation Mixture:
* 5.5 uL water * 1 uL ligation buffer * 0.5 uL ligase * 1 uL backbone (pAC997) * 2 uL insert
2. Let ligate at room temp for 30 minutes 
Note: For the control, add 2 uL of water instead of the insert
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