IGEM:UC Berkeley/2006/Ligation: Difference between revisions
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* 2 uL insert <br> | * 2 uL insert <br> | ||
2. Let ligate at room temp for 30 minutes <br> | |||
Note: For the control, add 2 uL of water instead of the insert | Note: For the control, add 2 uL of water instead of the insert | ||
Revision as of 15:04, 19 June 2006
1. Create Ligation Mixture:
* 5.5 uL water * 1 uL ligation buffer * 0.5 uL ligase * 1 uL backbone (pAC997) * 2 uL insert
2. Let ligate at room temp for 30 minutes
Note: For the control, add 2 uL of water instead of the insert
